Agilent quikchange lightning site directed mutagenesis kit - Download elena gheorghe your captain tonight
My vector is pDEST26 ( 7. I' ve tried lots of. Agilent quikchange lightning site directed mutagenesis kit. 3kb) target gene is 3k so total gene is about 12kb.
The Agilent SureCycler 8800 is one easy decision to a complete portfolio of PCR solutions SURELY BETTER PCR Easy interchangable module Gradient enables single run.
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QuikChange Lightning Site- Directed Mutagenesis Kit. Instruction Manual. Catalog # reactions) and # reactions) Revision F. The Q5 Site- Directed Mutagenesis Kit enables rapid, site- specific mutagenesis of double- stranded plasmid DNA in less than 2 hours ( Figure 1).
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The kit utilizes the robust Q5 Hot Start High- Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. I' ve done mutagenesis for 3 month, but until now all of my colonies have been only wild type.